Laboratory of Immunobiology of Inflammation, Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
Infectious Diseases and Tropical Medicine Post-Graduate Program, School of Medicine, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Front Cell Infect Microbiol. 2023 Mar 24;13:1143360. doi: 10.3389/fcimb.2023.1143360. eCollection 2023.
Chagas' disease is a tropical neglected illness caused by Trypanosoma cruzi and remains one of the most significant causes of morbidity and mortality in South and Central Americas. The disease is caused by a moderate to intense and persistent inflammatory response characterized by local upregulated expression and production of inflammatory mediators that favors the activation and recruitment of distinct cells of the immune system into different tissues to eliminate the parasites. Theracurmin is a curcumin's derived formulation of nanoparticles. Its anti-inflammatory properties make this bioactive compound a mitigating factor in pathological cases after an overwhelming inflammatory response.
Our research focused on the testicular investigation in 28 mice infected by 103 trypomastigote forms of Colombian strain of T. cruzi and preventively treated with Theracurmin. The mice were treated with 30 mg/Kg of Theracurmin during the period of 30 days. At the 30th day post infection animals were euthanized, and its testicles were collected to morphological and immunological assays.
The animals infected and treated with Theracurmin presented a reduction in the testicular levels of IL-15 and IL-6. The volume density (%) of the tunica propria was also higher in all infected animals, but Theracurmin decreased this parameter in the treated animals. In the intertubular area, the percentage of some intertubular components was decreased in the infected animals such as the percentage and volume of Leydig cells, connective tissue, and macrophages.
Furthermore, our data pointed to the daily use of Theracurmin in the diet as a protective element of the testicular function.
恰加斯病是一种由克氏锥虫引起的热带被忽视疾病，仍然是南美洲和中美洲发病率和死亡率的主要原因之一。这种疾病是由中度到高强度和持续的炎症反应引起的，其特征是局部上调表达和产生炎症介质，有利于免疫系统的不同细胞激活和募集到不同的组织中，以消除寄生虫。Theracurmin 是姜黄素的纳米粒子制剂。其抗炎特性使这种生物活性化合物成为过度炎症反应后病理性病例的缓解因素。
我们的研究集中在 28 只感染了哥伦比亚株 T. cruzi 的 103 个锥虫形式的睾丸研究，并预防性地用 Theracurmin 治疗。这些小鼠在 30 天的时间内用 30mg/kg 的 Theracurmin 治疗。在感染后第 30 天，动物被安乐死，收集其睾丸进行形态学和免疫学检测。
感染并接受 Theracurmin 治疗的动物睾丸中 IL-15 和 IL-6 的水平降低。所有感染动物的固有层体积密度（%）也更高，但 Theracurmin 降低了治疗动物的这一参数。在间质区域，一些间质成分的百分比在感染动物中降低，如间质细胞、结缔组织和巨噬细胞的百分比和体积。
此外，我们的数据表明，每天在饮食中使用 Theracurmin 作为睾丸功能的保护因素。
