Istanbul Technical University, Faculty of Science and Letters, Department of Chemistry, Maslak 34469 Istanbul, Turkey.
Macromol Biosci. 2011 Jan 10;11(1):141-50. doi: 10.1002/mabi.201000280. Epub 2010 Oct 13.
Hydrophobic- and/or hydrophilic-polymer-grafted PDVB microspheres are synthesized by the combination of hydrobromination and click-chemistry processes. The modified-PDVB microspheres and the intermediates at various stages of synthesis are characterized using GPC, ¹H NMR and FTIR spectroscopy and TGA analysis. Use of the microspheres as a support matrix for reversible protein immobilization via adsorption is investigated. The system parameters such as the adsorption conditions (i.e., enzyme concentration, medium pH) and desorption are studied and evaluated with regards to the biocatalytic activity and adsorption capacity.
通过氢溴化和点击化学过程的结合，合成了疏水和亲水聚合物接枝 PDVB 微球。使用 GPC、¹H NMR 和 FTIR 光谱以及 TGA 分析对改性-PDVB 微球和合成各阶段的中间体进行了表征。研究了将微球用作通过吸附固定化可逆蛋白质的支撑基质。研究并评估了系统参数，如吸附条件（即酶浓度、介质 pH）和洗脱条件，以考察生物催化活性和吸附容量。
