间日疟原虫环子孢子蛋白在松鼠猴体内的检测
Testing of Plasmodium vivax CS proteins in Saimiri monkeys.
作者信息
Collins W E
机构信息
Malaria Branch, Centers for Disease Control, Atlanta, GA 30333.
出版信息
Bull World Health Organ. 1990;68 Suppl(Suppl):42-6.
Recombinant proteins directed against the circumsporozoite (CS) protein of Plasmodium vivax were tested for safety, immunogenicity, and efficacy in Saimiri sciureus boliviensis monkeys. The monkeys were immunized 3 times with either alum or muramyl tripeptide as adjuvant, and were challenged with 10,000 sporozoites (P. vivax, Salvador I strain) from mosquitos infected by membrane feeding on gametocytes from chimpanzees. Antibodies, measured by indirect immunofluorescent antibody tests and enzyme-linked immunosorbent assays, were induced by all the vaccines. Six of 42 monkeys immunized with recombinant or synthetically produced vaccines were protected. Two of 6 monkeys immunized with irradiated sporozoites were protected. Saimiri appear to be less susceptible than are humans to sporozoite infection. Therefore, the predictive usefulness of this model must await the results of comparable trials in monkeys and humans.
针对间日疟原虫环子孢子（CS）蛋白的重组蛋白在松鼠猴（Saimiri sciureus boliviensis）中进行了安全性、免疫原性和有效性测试。这些猴子用明矾或胞壁酰三肽作为佐剂进行了3次免疫，并受到来自通过膜饲接种感染黑猩猩配子体的蚊子的10000个疟原虫子孢子（间日疟原虫，萨尔瓦多I株）的攻击。通过间接免疫荧光抗体试验和酶联免疫吸附测定法测量的抗体由所有疫苗诱导产生。用重组或合成生产的疫苗免疫的42只猴子中有6只受到了保护。用辐照子孢子免疫的6只猴子中有2只受到了保护。松鼠猴似乎比人类对疟原虫子孢子感染的易感性更低。因此，该模型的预测有用性必须等待在猴子和人类中进行的类似试验的结果。
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