基于碳纳米管修饰丝网印刷电极的生物传感器对谷氨酸的纳摩尔级检测
Nanomolar Detection of Glutamate at a Biosensor Based on Screen-Printed Electrodes Modified with Carbon Nanotubes.
作者信息
Khan Raju, Gorski Waldemar, Garcia Carlos D
机构信息
Analytical Chemistry Division, North East Institute of Science & Technology, Jorhat, 785006, Assam, India.
出版信息
Electroanalysis. 2011 Oct;23(10):2357-2363. doi: 10.1002/elan.201100348. Epub 2011 Sep 20.
Abstract
The amperometric glutamate biosensor based on screen-printed electrodes containing carbon nanotubes (CNT), and its integration in a flow injection analysis system, is described herein. The sensor was fabricated by simply adsorbing enzyme glutamate oxidase (GlutOx) on a commercial substrate containing multi-wall CNT. The resulting device displayed excellent electroanalytical properties toward the determination of L-glutamate in a wide linear range (0.01-10 μM) with low detection limit (10 nM, S/N≥3), fast response time (≤5 s), and good operational and long-term stability. The CNT modified screen-printed electrodes have a potential to be of general interest for designing of electrochemical sensors and biosensors.
摘要
本文描述了基于含有碳纳米管（CNT）的丝网印刷电极的安培型谷氨酸生物传感器及其在流动注射分析系统中的集成。该传感器通过将谷氨酸氧化酶（GlutOx）简单吸附在含有多壁碳纳米管的商业基底上制备而成。所得装置在宽线性范围（0.01 - 10 μM）内对L - 谷氨酸的测定表现出优异的电分析性能，检测限低（10 nM，S/N≥3），响应时间快（≤5 s），且具有良好的操作稳定性和长期稳定性。碳纳米管修饰的丝网印刷电极在设计电化学传感器和生物传感器方面具有广泛的应用潜力。
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