使用E-MAP方法进行定量遗传相互作用图谱分析。
Quantitative genetic interaction mapping using the E-MAP approach.
作者信息
Collins Sean R, Roguev Assen, Krogan Nevan J
机构信息
Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California, USA.
出版信息
Methods Enzymol. 2010;470:205-31. doi: 10.1016/S0076-6879(10)70009-4. Epub 2010 Mar 1.
Abstract
Genetic interactions represent the degree to which the presence of one mutation modulates the phenotype of a second mutation. In recent years, approaches for measuring genetic interactions systematically and quantitatively have proven to be effective tools for unbiased characterization of gene function and have provided valuable data for analyses of evolution. Here, we present protocols for systematic measurement of genetic interactions with respect to organismal growth rate for two yeast species.
摘要
基因相互作用指的是一种突变的存在对另一种突变表型的调节程度。近年来，系统且定量地测量基因相互作用的方法已被证明是用于无偏见地表征基因功能的有效工具，并为进化分析提供了有价值的数据。在此，我们展示了针对两种酵母物种，就其生长速率系统测量基因相互作用的实验方案。
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