Broin S O, Clarke E, McCann S
Department of Haematology, St. James's Hospital, Dublin.
Ir J Med Sci. 1990 Sep-Dec;159(9-12):269-71. doi: 10.1007/BF02993609.
Factors which can account for the poor correlation between whole blood and plasma Cyclosporine (CsA) levels in patients on CsA prophylaxis are evaluated. The study took account of the influence of plasma separation procedures, and the sample haematocrit on CsA distribution in the blood of renal transplant patients (n = 35). CsA was measured using both specific and non-specific CsA radioimmunoassays. Significant negative correlations occurred between CsA distribution and the haematocrit, independently of the plasma separation procedure or the specificity of the assay. All results were lower when using the specific assay but a significantly higher percentage of CsA was measured in the plasma by specific assay compared to nonspecific assay when plasma was separated at both 22 degrees C (t-test, p less than 0.02) and at 37 degrees C, p less than 0.01). This may relate to the selective binding of CsA and its analogues by blood cells. This study is a prelude to the development of more consistent plasma separation procedures in the monitoring of this drug.
对环孢素（CsA）预防治疗患者全血与血浆中环孢素（CsA）水平相关性较差的影响因素进行了评估。该研究考虑了血浆分离程序以及样本血细胞比容对肾移植患者（n = 35）血液中CsA分布的影响。使用特异性和非特异性CsA放射免疫测定法对CsA进行测量。CsA分布与血细胞比容之间存在显著的负相关，与血浆分离程序或测定的特异性无关。使用特异性测定法时所有结果均较低，但当在22℃（t检验，p < 0.02）和37℃（p < 0.01）分离血浆时，与非特异性测定法相比，特异性测定法在血浆中测得的CsA百分比显著更高。这可能与血细胞对CsA及其类似物的选择性结合有关。本研究是开发在该药物监测中更一致的血浆分离程序的前奏。
