Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Cell. 2010 Oct 15;143(2):191-200. doi: 10.1016/j.cell.2010.09.031.
Protein splicing is a naturally occurring process in which a protein editor, called an intein, performs a molecular disappearing act by cutting itself out of a host protein in a traceless manner. In the two decades since its discovery, protein splicing has been harnessed for the development of several protein-engineering methods. Collectively, these technologies help bridge the fields of chemistry and biology, allowing hitherto impossible manipulations of protein covalent structure. These tools and their application are the subject of this Primer.
蛋白质剪接是一种自然发生的过程,其中一种称为内含肽的蛋白质编辑酶以无痕的方式将自身从宿主蛋白中切割出来。自发现以来的二十年中,蛋白质剪接已被用于开发几种蛋白质工程方法。这些技术共同架起了化学和生物学之间的桥梁,使人们以前无法实现的对蛋白质共价结构的操作成为可能。这些工具及其应用是本篇综述的主题。