Minnihan Ellen C, Yokoyama Kenichi, Stubbe Joanne
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307, USA.
F1000 Biol Rep. 2009 Nov 26;1:88. doi: 10.3410/B1-88.
Considerable effort has been dedicated to the development of technology for the site-specific incorporation of unnatural amino acids into proteins, with nonsense codon suppression and expressed protein ligation emerging as two of the most promising methods. Recent research advances in which these methods have been applied to study protein function and mechanism are briefly highlighted, and the potential of the methods for efficient, widespread future use in vitro and in vivo is critically evaluated.
人们已经付出了巨大努力来开发将非天然氨基酸位点特异性地掺入蛋白质中的技术，其中无义密码子抑制和表达蛋白连接已成为最有前景的两种方法。本文简要介绍了这些方法在研究蛋白质功能和机制方面的最新研究进展，并对这些方法在未来体外和体内高效、广泛应用的潜力进行了批判性评估。
