Lee Byeong Sung, Shin Seunggun, Jeon Jong Yeob, Jang Kyoung-Soon, Lee Bun Yeol, Choi Sangdun, Yoo Tae Hyeon
§Division of Mass Spectrometry Research, Korea Basic Science Institute, 162 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju 363-883, Korea.
ACS Chem Biol. 2015 Jul 17;10(7):1648-53. doi: 10.1021/acschembio.5b00230. Epub 2015 May 12.
The biological protein synthesis system has been engineered to incorporate unnatural amino acid into proteins, and this has opened up new routes for engineering proteins with novel compositions. While such systems have been successfully applied in research, there remains a need to develop new approaches with respect to the wider application of unnatural amino acids. In this study, we reported a strategy for incorporating unnatural amino acids into proteins by reassigning one of the Arg sense codons, the AGG codon. Using this method, several unnatural amino acids were quantitatively incorporated into the AGG site. Furthermore, we applied the method to multiple AGG sites, and even to tandem AGG sequences. The method developed and described here could be used for engineering proteins with diverse unnatural amino acids, particularly when employed in combination with other methods.
生物蛋白质合成系统已被设计用于将非天然氨基酸掺入蛋白质中，这为构建具有新型组成的蛋白质开辟了新途径。虽然此类系统已在研究中成功应用，但仍需要开发新方法以实现非天然氨基酸更广泛的应用。在本研究中，我们报道了一种通过重新分配精氨酸（Arg）的一个有义密码子（AGG密码子）将非天然氨基酸掺入蛋白质的策略。使用该方法，几种非天然氨基酸被定量掺入到AGG位点。此外，我们将该方法应用于多个AGG位点，甚至串联的AGG序列。本文所开发和描述的方法可用于构建含有多种非天然氨基酸的蛋白质，特别是与其他方法结合使用时。
