Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, West Bengal, India.
Biotechnol J. 2010 Nov;5(11):1181-5. doi: 10.1002/biot.201000252.
Poly-β-hydroxybutyrate (PHB) is a biodegradable and biocompatible polymer that has immense potential in the field of environmental, agricultural and biomedical sciences. An alternative host system has been explored in this study for low-cost production. Examination of 25 cyanobacterial species from 19 different genera for photoautrophic production of polyhydroxyalkanoates (PHAs) under batch culture demonstrated that 20 species were poly-β-hydroxybutyrate (PHB) accumulators, while others were found to be negative. Presence of PHB was confirmed by UV-spectroscopy, (1)H-NMR spectroscopy and GC-MS analysis. Accumulation of PHB in cyanobacteria was found to be species specific. The PHB extracted from Nostoc muscorum exhibited comparable material properties with the commercial PHB, thus advocating its potential applications in various fields.
聚-β-羟基丁酸酯（PHB）是一种可生物降解和生物相容的聚合物，在环境、农业和生物医学科学领域具有巨大的潜力。本研究探索了一种替代的宿主系统，以实现低成本生产。在分批培养中，对来自 19 个不同属的 25 种蓝藻进行了考察，以研究其对聚羟基烷酸酯（PHA）的光自养生产能力。结果表明，有 20 种蓝藻是聚-β-羟基丁酸酯（PHB）的积累者，而其他的则为阴性。通过紫外光谱、（1）H-NMR 光谱和 GC-MS 分析证实了 PHB 的存在。发现 PHB 在蓝藻中的积累具有种特异性。从念珠藻中提取的 PHB 表现出与商业 PHB 相当的材料性能，因此提倡其在各个领域的潜在应用。
