Huang Naiyan, Wang Hequn, Zhao Jianhua, Lui Harvey, Korbelik Mladen, Zeng Haishan
Cancer Imaging Department, British Columbia Cancer Research Centre, 675 West 10th Avenue, Vancouver, BC, Canada V5Z 1L3.
Lasers Surg Med. 2010 Nov;42(9):638-48. doi: 10.1002/lsm.20968.
There has been a dramatic increase in photothermal therapy as a minimally invasive treatment modality for cancer treatment due to the development of novel nanomaterials as the light absorption agents. Single-wall carbon nanotubes (SWNTs) with strong optical absorption in the broad visible and near IR offer unique advantages for photothermal cancer therapy. A broad range of wavelengths can be used for the treatment with SWNTs, whereas conventional photothermal therapeutic agent is designed to absorb light only near one selected wavelength. The objective of this study is to validate the hypothesis that intratumoral injected SWNTs can absorb 785 nm near IR laser light and generate significant local hyperthermia to destroy tumors.
STUDY DESIGN/MATERIALS AND METHODS: SCCVII tumor in C3H/HeN mice was exposed to 785-nm laser after intratumoral injection of SWNTs with different light and SWNTs dose combinations. The temperatures of the tumor with laser irradiation were monitored. In vivo and ex vivo Raman spectra in different organs were obtained with a rapid Raman system. Tumor responses (tumor volume and mouse survival) were documented daily after treatment up to day 45 to assess the effectiveness of the treatment.
The temperature within the tumors increased in a light- and SWNTs-dose dependent manner. Squamous cell carcinomas can be eradicated at a moderate light irradiance and fluence (200 mW/cm² and 120 J/cm²). This light dose is also comparable to those used with photodynamic therapy. Tissue Raman spectroscopy measurements revealed that SWNTs remained localized in the tumor even 3 months after injection but was not found in other organs.
This animal study represents a significant step forward towards the goal of advancing SWNTs based photothermal cancer therapy into clinical applications.
由于新型纳米材料作为光吸收剂的发展，光热疗法作为一种用于癌症治疗的微创治疗方式有了显著增长。单壁碳纳米管（SWNTs）在宽可见光和近红外区域具有强光学吸收，为光热癌症治疗提供了独特优势。SWNTs可使用广泛的波长进行治疗，而传统光热治疗剂仅设计用于在一个选定波长附近吸收光。本研究的目的是验证以下假设：瘤内注射SWNTs可吸收785nm近红外激光并产生显著的局部热疗以破坏肿瘤。
研究设计/材料与方法：在C3H/HeN小鼠的SCCVII肿瘤内注射不同光和SWNTs剂量组合后，将其暴露于785nm激光下。监测激光照射时肿瘤的温度。使用快速拉曼系统获得不同器官的体内和体外拉曼光谱。治疗后每天记录肿瘤反应（肿瘤体积和小鼠存活情况）直至第45天，以评估治疗效果。
肿瘤内的温度以光和SWNTs剂量依赖性方式升高。在中等光辐照度和能量密度（200mW/cm²和120J/cm²）下可根除鳞状细胞癌。该光剂量也与光动力疗法所用的剂量相当。组织拉曼光谱测量显示，即使在注射后3个月，SWNTs仍局限于肿瘤中，但在其他器官中未发现。
这项动物研究朝着将基于SWNTs的光热癌症治疗推进到临床应用的目标迈出了重要一步。
