Biocontrol and Biosecurity, AgResearch, Lincoln Research Centre, Private Bag 4749, Christchurch 8140, New Zealand.
Plasmid. 2011 Jan;65(1):32-41. doi: 10.1016/j.plasmid.2010.10.001. Epub 2010 Oct 13.
Some strains of Serratia entomophila and S. proteamaculans cause amber disease of the New Zealand grass grub Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The disease determinants of S. entomophila, are encoded on a 153,404-bp plasmid, termed pADAP for amber disease associated plasmid. The S. proteamaculans strain 143 (Sp143) exhibits an unusual pathotype, where only 60-70% of C. zealandica larvae infected with the bacterium succumb to disease. DNA sequence analysis of the Sp143 pU143 virulence associated region identified high DNA similarity to the pADAP sep virulence associated region, with DNA sequence variation in the sepA gene and the variable region of the sepC component. No pADAP anti-feeding prophage orthologue was detected in the Sp143 genome. The region of pADAP replication was cloned and found to replicate in S. entomophila but not in Escherichia coli. DNA sequence analysis of the plasmid pSG348 repA gene from the French isolate of Serratia grimesii, identified 93% DNA identity to the pADAP repA gene. A comparison of the pU143 virulence associated region with the completed pADAP nucleotide sequence is given.
有些菌株的嗜虫沙雷氏菌和 S. proteamaculans 会导致新西兰草地幼虫（Costelytra zealandica）（鞘翅目：金龟子科）的琥珀病，这是新西兰一种重要的牧场害虫。嗜虫沙雷氏菌的疾病决定因素编码在一个 153404bp 的质粒上，称为与琥珀病相关的质粒 pADAP。S. proteamaculans 菌株 143（Sp143）表现出一种不寻常的病理型，只有 60-70%感染该细菌的 C. zealandica 幼虫会屈服于疾病。对 Sp143 pU143 毒力相关区域的 DNA 序列分析表明，它与 pADAP sep 毒力相关区域具有高度的 DNA 相似性，sepA 基因和 sepC 成分的可变区存在 DNA 序列变异。在 Sp143 基因组中未检测到 pADAP 抗食原噬菌体的同源物。pADAP 复制区被克隆，并发现它在嗜虫沙雷氏菌中复制，但不在大肠杆菌中复制。对来自法国的沙雷氏菌属格里姆氏分离株的质粒 pSG348 repA 基因的 DNA 序列分析表明，它与 pADAP repA 基因的 DNA 同一性为 93%。给出了 pU143 毒力相关区域与完整的 pADAP 核苷酸序列的比较。
