Dodd Steven J, Hurst Mark R H, Glare Travis R, O'Callaghan Maureen, Ronson Clive W
Biocontrol and Biosecurity, AgResearch, PO Box 60, Lincoln, New Zealand.
Appl Environ Microbiol. 2006 Oct;72(10):6584-92. doi: 10.1128/AEM.00954-06.
Some strains of Serratia entomophila and S. proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. Sequence analysis suggests that the sepABC gene cluster may be part of a horizontally mobile region. This study presents evidence for the putative mobility of the sep genes of pADAP. Southern blot analysis showed that orthologues of the sep genes reside on plasmids within S. entomophila, S. liquefaciens, S. proteamaculans, and a plasmid from Yersinia frederiksenii. Three plasmids hybridized to the pADAP sep virulence-associated region but not the pADAP replication and conjugation regions. Subsequent DNA sequence analysis of the Y. frederiksenii sep-like genes, designated tcYF1 and tcYF2, showed that they had 88% and 87% DNA identity to sepA and sepB, respectively. These results indicate that the sep genes are part of a discrete horizontally mobile region.
某些嗜虫沙雷氏菌和变形斑沙雷氏菌菌株会引发新西兰伪步行虫（鞘翅目：金龟子科）的琥珀病。毒力所需的三个基因，即sepABC，位于一个大质粒pADAP上。序列分析表明，sepABC基因簇可能是水平移动区域的一部分。本研究提供了pADAP的sep基因具有假定移动性的证据。Southern印迹分析表明，sep基因的直系同源物存在于嗜虫沙雷氏菌、液化沙雷氏菌、变形斑沙雷氏菌的质粒中，以及来自费氏耶尔森菌的一个质粒上。三个质粒与pADAP的sep毒力相关区域杂交，但不与pADAP的复制和接合区域杂交。随后对费氏耶尔森菌的sep样基因（命名为tcYF1和tcYF2）进行的DNA序列分析表明，它们与sepA和sepB的DNA同一性分别为88%和87%。这些结果表明，sep基因是一个离散的水平移动区域的一部分。
