Department of Oral and Maxillofacial Sciences, University of Naples "Federico II", Napoli, Italy.
J Endod. 2010 Nov;36(11):1839-43. doi: 10.1016/j.joen.2010.08.010. Epub 2010 Sep 19.
Mineral trioxide aggregate (MTA) is known to stimulate the hard tissue repair process. The purpose of this study was to evaluate the ability of MTA to support the adhesion, proliferation, and migration of human bone marrow-derived mesenchymal stem cells (hMSCs).
White ProRoot MTA and white Portland cement were mixed and left to set 24 hours. MSCs were cultured on the samples and observed after 24 hours by confocal laser scanning microscopy (CLSM) by using the cytoskeleton marker CellTracker. Cell proliferation was evaluated by means of alamar blue assay in the presence and absence of differentiation medium during a period of 28 days, and cells seeded on polystyrene culture wells were the control. To assess the effect on migratory ability of hMSCs, a transwell migration assay was performed for 18 hours, positioning MTA and Portland cement in 6-well plates and the cells in 8-μm pore inserts.
hMSCs observed under CLSM showed attachment and spread activity on the upper surface of the MTA. Cell proliferation was significantly higher on MTA than on Portland cement. A rate proliferation increase of the MTA group compared with the control was observed after 14 days in presence of basic medium, whereas the same effect was reached after 21 days in presence of differentiation medium. Moreover, MTA was able to enhance cell migration significantly more than Portland cement.
Our findings suggest that MTA was able to assist hMSC adhesion, growth, and migration.
矿化三氧化物凝聚体（MTA）被认为能刺激硬组织修复过程。本研究旨在评估 MTA 支持人骨髓间充质干细胞（hMSCs）黏附、增殖和迁移的能力。
白色 ProRoot MTA 和白色波特兰水泥混合并放置 24 小时凝固。将 MSCs 接种在样本上，24 小时后通过使用细胞骨架标记物 CellTracker 的共聚焦激光扫描显微镜（CLSM）进行观察。通过在存在和不存在分化培养基的情况下在 28 天内使用 alamar blue 测定法评估细胞增殖，接种在聚苯乙烯培养孔中的细胞作为对照。为了评估对 hMSCs 迁移能力的影响，进行了 18 小时的 Transwell 迁移测定，将 MTA 和波特兰水泥置于 6 孔板中，将细胞置于 8μm 孔滤器中。
CLSM 下观察到的 hMSCs 在 MTA 的上表面显示出附着和扩展活性。与 Portland 水泥相比，MTA 上的细胞增殖明显更高。在碱性培养基中，MTA 组与对照组相比，在第 14 天观察到增殖率增加，而在分化培养基中，同样的效果在第 21 天达到。此外，MTA 能够显著增强细胞迁移，比 Portland 水泥更有效。
我们的研究结果表明，MTA 能够辅助 hMSC 黏附、生长和迁移。
