Takita T, Hayashi M, Takeichi O, Ogiso B, Suzuki N, Otsuka K, Ito K
Department of Endodontics, Nihon University School of Dentistry, Chiyoda-ku, Tokyo, Japan.
Int Endod J. 2006 May;39(5):415-22. doi: 10.1111/j.1365-2591.2006.01097.x.
To investigate the effect of mineral trioxide aggregate (MTA) on the proliferation of human dental pulp (HDP) cells ex-vivo.
Human dental pulp cells were cultured with MTA or calcium hydroxide-containing cement (Dycal) using culture plate inserts. Control cells were cultured with culture plate inserts only. Cell proliferation was measured for up to 14 days using a Cell Counting kit, and the concentration of calcium ions released from the tested materials was assessed using a Calcium E-test kit. To confirm that the effect of MTA was attributable to released calcium ions, cell proliferation was measured in the presence of exogenous calcium chloride as a source of calcium ions while in the absence of MTA.
Mineral trioxide aggregate significantly stimulated cell proliferation after 12 days, whereas Dycal had no such effect. The number of calcium ions released from MTA was significantly higher than that released from Dycal. Following the addition of calcium chloride, cell proliferation increased in a dose-dependent manner after 12 days. Moreover, cell proliferation showed a similar pattern whether a given concentration of calcium ions was produced by calcium chloride or by release from MTA.
In this ex-vivo study, the elution components such as calcium ions from MTA had higher proliferation ability of HDP cells than control and Dycal.
研究三氧化矿物凝聚体（MTA）对人牙髓（HDP）细胞体外增殖的影响。
使用培养板插入物将人牙髓细胞与MTA或含氢氧化钙的水门汀（Dycal）一起培养。对照细胞仅用培养板插入物培养。使用细胞计数试剂盒测量长达14天的细胞增殖，并使用钙E测试试剂盒评估从测试材料中释放的钙离子浓度。为了确认MTA的作用归因于释放的钙离子，在不存在MTA的情况下，以氯化钙作为钙离子来源测量细胞增殖。
三氧化矿物凝聚体在12天后显著刺激细胞增殖，而Dycal没有这种作用。从MTA释放的钙离子数量显著高于从Dycal释放的钙离子数量。添加氯化钙后，12天后细胞增殖呈剂量依赖性增加。此外，无论给定浓度的钙离子是由氯化钙产生还是由MTA释放产生，细胞增殖都呈现相似的模式。
在这项体外研究中，MTA洗脱成分如钙离子对HDP细胞的增殖能力高于对照和Dycal。
