Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2011 Jan;102(2):1663-8. doi: 10.1016/j.biortech.2010.09.083. Epub 2010 Sep 27.
In order to realize the separated ethanol fermentation of glucose and xylose, prehydrolysis of corn stover with sulfuric acid at moderate temperature was applied, while inhibitors were produced inevitably. A complex extraction was adopted to detoxify the prehydrolyzate before fermentation to ethanol with Pichia stipitis CBS 5776. The best proportion of mixed extractant was 30% trialkylamine-50% n-octanol -20% kerosene. Detoxification results indicated that 73.3% of acetic acid, 45.7% of 5-hydroxymethylfurfural and 100% of furfural could be removed. Compared with the undetoxified prehydrolyzate, the fermentability of the detoxified prehydrolyzate was significantly improved. After 48 h fermentation of the detoxified prehydrolyzate containing 7.80 g/l of glucose and 52.8 g/l of xylose, the sugar utilization ratio was 93.2%; the ethanol concentration reached its peak value of 21.8 g/l, which was corresponding to 82.3% of the theoretical value.
为了实现葡萄糖和木糖的分别乙醇发酵，采用硫酸对玉米秸秆进行中温预水解，不可避免地会产生抑制剂。采用复合萃取法对 Pichia stipitis CBS 5776 发酵前的预水解物进行乙醇脱毒。最佳混合萃取剂比例为 30%三烷基胺-50%正辛醇-20%煤油。脱毒结果表明，73.3%的乙酸、45.7%的 5-羟甲基糠醛和 100%的糠醛均可去除。与未经脱毒的预水解物相比，脱毒预水解物的发酵性能显著提高。在 7.80 g/L 葡萄糖和 52.8 g/L 木糖的脱毒预水解物中发酵 48 h 后，糖利用率达到 93.2%；乙醇浓度达到 21.8 g/L 的峰值，对应于理论值的 82.3%。
