Zhu Junjun, Yang Jinlong, Zhu Yuanyuan, Zhang Lingling, Yong Qiang, Xu Yong, Li Xin, Yu Shiyuan
College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China,
Bioprocess Biosyst Eng. 2014 Nov;37(11):2215-22. doi: 10.1007/s00449-014-1199-0. Epub 2014 May 6.
The prehydrolyzate obtained from acid-catalyzed steam-exploded corn stover (ASC) mainly contains xylose and a number of inhibitory compounds that inhibit ethanol fermentation by Pichia stipitis. In this study, the effects of the ASC prehydrolyzate, specifically those of the carbohydrate-degradation products, lignin-degradation products (which were extracted from ASC prehydrolyzate using ethyl acetate), and six major phenolic compounds (added to pure-sugar media individually or in combination), on ethanol fermentation were investigated. Results indicate that the effects of the carbohydrate-degradation products were negligible (10 h delayed) compared with those of pure-sugar fermentation, whereas the effects of the lignin-degradation products were significant (52 h delayed). Meanwhile, the inhibitory effects of the major phenolic compounds were not caused by certain types of inhibitors, but were due to the synergistic effects of various inhibitors.
由酸催化蒸汽爆破玉米秸秆（ASC）得到的预水解产物主要含有木糖和多种抑制树干毕赤酵母乙醇发酵的抑制性化合物。在本研究中，考察了ASC预水解产物，特别是碳水化合物降解产物、木质素降解产物（用乙酸乙酯从ASC预水解产物中提取）以及六种主要酚类化合物（单独或组合添加到纯糖培养基中）对乙醇发酵的影响。结果表明，与纯糖发酵相比，碳水化合物降解产物的影响可忽略不计（延迟10小时），而木质素降解产物的影响显著（延迟52小时）。同时，主要酚类化合物的抑制作用并非由某些类型的抑制剂引起，而是由于各种抑制剂的协同作用。
