系统性红斑狼疮患者血清和尿液中的细胞游离 miR-146a 和 miR-155。

Serum and urinary cell-free MiR-146a and MiR-155 in patients with systemic lupus erythematosus.

机构信息

Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.

出版信息

J Rheumatol. 2010 Dec;37(12):2516-22. doi: 10.3899/jrheum.100308. Epub 2010 Oct 15.

DOI:10.3899/jrheum.100308

PMID:20952466

Abstract

OBJECTIVE

Recent studies showed that micro-RNA play important roles in the pathogenesis of autoimmune diseases. We studied the levels of miR-146a and miR-155 in the serum and urinary supernatant of patients with systemic lupus erythematosus (SLE).

METHODS

The serum and urinary supernatant levels of miR-146a and miR-155 were determined by real-time quantitative polymerase chain reaction in 40 patients with SLE and 30 healthy controls.

RESULTS

Compared to controls, serum miR-146a and miR-155 levels were lower, and the urinary level of miR-146a was higher, in SLE. Estimated glomerular filtration rate (eGFR) correlated with both serum miR-146a (r = 0.519, p = 0.001) and miR-155 (r = 0.384, p = 0.014). Serum miR-146a inversely correlated with proteinuria (r = -0.341, p = 0.031) and the SLE Disease Activity Index (r = -0.465, p = 0.003). Serum miR-146a and miR-155 levels also correlated with red blood cell count, platelet count, and lymphocyte count. After treatment with calcitriol for 6 months, serum miR-146a level of SLE patients increased significantly (p < 0.001), and its change inversely correlated with the level of calcium-phosphate product (r = -0.466, p = 0.003).

CONCLUSION

The results suggested that serum miR-146a and miR-155 participate in the pathophysiology of SLE and might be used as biomarkers of SLE.

摘要

目的

最近的研究表明，微小 RNA 在自身免疫性疾病的发病机制中发挥重要作用。我们研究了系统性红斑狼疮（SLE）患者血清和尿上清液中 miR-146a 和 miR-155 的水平。

方法

通过实时定量聚合酶链反应测定 40 例 SLE 患者和 30 例健康对照者血清和尿上清液中 miR-146a 和 miR-155 的水平。

结果

与对照组相比，SLE 患者血清 miR-146a 和 miR-155 水平降低，尿 miR-146a 水平升高。估计肾小球滤过率（eGFR）与血清 miR-146a（r = 0.519，p = 0.001）和 miR-155（r = 0.384，p = 0.014）相关。血清 miR-146a 与蛋白尿（r = -0.341，p = 0.031）和 SLE 疾病活动指数（r = -0.465，p = 0.003）呈负相关。血清 miR-146a 和 miR-155 水平也与红细胞计数、血小板计数和淋巴细胞计数相关。用骨化三醇治疗 6 个月后，SLE 患者血清 miR-146a 水平显著升高（p < 0.001），其变化与钙磷乘积呈负相关（r = -0.466，p = 0.003）。