Department of Osteoporosis and Bone Diseases, The Shanghai Sixth People's Hospital, Shanghai Jiaotong University, China.
Acta Pharmacol Sin. 2010 Nov;31(11):1464-9. doi: 10.1038/aps.2010.92. Epub 2010 Oct 18.
To investigate the effect of low-density lipoprotein receptor-related protein 5 (LRP5) gene polymorphisms on bone and obesity phenotypes in young Chinese men.
A total of 1244 subjects from 411 Chinese nuclear families were genotyped by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique at the Q89R, N740N, and A1330V sites in the LRP5 gene. Bone mineral density (BMD) in the lumbar spine and the hip, total fat mass and total lean mass were measured using dual-energy X-ray absorptiometry. The association between LRP5 gene polymorphisms and peak BMD, body mass index (BMI), total fat mass, total lean mass and percentage of fat mass was assessed using a quantitative transmission disequilibrium test (QTDT).
No significant within-family associations were found between genotypes or haplotypes of the LRP5 gene and peak BMD, BMI, total fat mass, total lean mass and percentage of fat mass. The 1000 permutations that were subsequently simulated were in agreement with these within-family association results.
Our results suggest that common polymorphic variations of the LRP5 gene do not influence peak bone mass acquisition and obesity phenotypes in young Chinese men.
探讨低密度脂蛋白受体相关蛋白 5（LRP5）基因多态性对中国年轻男性骨和肥胖表型的影响。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对 411 个中国核心家庭的 1244 名受试者的 LRP5 基因 Q89R、N740N 和 A1330V 位点进行基因分型。采用双能 X 射线吸收法测量腰椎和髋部骨矿物质密度（BMD）、总脂肪量和总瘦体重。采用定量传递不平衡检验（QTDT）评估 LRP5 基因多态性与峰值 BMD、体重指数（BMI）、总脂肪量、总瘦体重和脂肪量百分比之间的关联。
LRP5 基因的基因型或单倍型与峰值 BMD、BMI、总脂肪量、总瘦体重和脂肪量百分比之间无显著的家系内关联。随后模拟的 1000 次随机抽样结果与这些家系内关联结果一致。
我们的结果表明，LRP5 基因常见的多态性变异并不影响中国年轻男性的峰值骨量获得和肥胖表型。
