Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto 14040-903, Brazil.
Evid Based Complement Alternat Med. 2011;2011. doi: 10.1155/2011/863917. Epub 2010 Sep 8.
This study investigated the potential use of topically and orally administered propolis extracts to prevent UV irradiation-induced oxidative stress in skin. The results illustrated that green propolis extract (GPE) contained greater amounts of polyphenols, coumaric acid, drupanin, baccharin and artepillin C than did brown propolis extract (BPE). GPE showed higher antioxidant activity than BPE when the IC(50) (concentration that caused 50% inhibition) values were compared. Interesting, the oral treatment of hairless mice demonstrated a recovery of 30.0% for GPE and 22.8% for BPE with respect to UV irradiation-induced GSH depletion. The topical pretreatment of animals with both propolis extract solutions recovered around 14.0% of the depleted GSH. However, the employed treatments did not inhibit the increase of cutaneous proteinase secretion/activity caused by irradiation. These findings indicate that despite differences in composition and antioxidant properties, GPE and BPE both successfully prevent UV-induced GSH depletion in vivo and are both promising antioxidant systems against oxidative stress in skin. Based on these findings, complementary studies should be performed to enhance our understanding of the protective effects of propolis extracts in skin.
本研究探讨了局部和口服施用蜂胶提取物预防皮肤紫外线照射诱导的氧化应激的潜力。结果表明，绿蜂胶提取物（GPE）比棕蜂胶提取物（BPE）含有更多的多酚、香豆酸、灵芝酸、巴卡丁和 Artepillin C。当比较 IC50（引起 50%抑制的浓度）值时，GPE 表现出比 BPE 更高的抗氧化活性。有趣的是，口服给予无毛小鼠 GPE 和 BPE 治疗，分别恢复了 30.0%和 22.8%的 GSH 耗竭，这是由 UV 照射引起的。两种蜂胶提取物溶液的局部预处理使耗竭的 GSH 恢复了约 14.0%。然而，所采用的治疗方法并没有抑制照射引起的皮肤蛋白酶分泌/活性的增加。这些发现表明，尽管组成和抗氧化特性存在差异，但 GPE 和 BPE 都能成功地预防体内 UV 诱导的 GSH 耗竭，并且都是对抗皮肤氧化应激的有前途的抗氧化系统。基于这些发现，应该进行补充研究，以增强我们对蜂胶提取物在皮肤中的保护作用的理解。
