Faculty of Pharmacy, University of Sydney, Sydney, NSW, Australia.
Int Arch Allergy Immunol. 2010;152(2):87-97. doi: 10.1159/000265530. Epub 2009 Dec 16.
Propolis is a honeybee product that has been used in traditional medicine for antioxidant, immune-stimulating, anti-inflammatory and anti-cancer effects. Here, the potential of the topical application of a crude ethanolic extract of Sydney propolis to protect against UV-radiation-induced impairments associated with an increased risk of photocarcinogenesis has been tested in the hairless mouse.
Solutions providing between 10 and 200 mg/kg propolis were applied to the skin following UV irradiation. The inflammation from exposure to UV (290-400 nm) was quantitated by measurement of increased skinfold thickness; lipid peroxidation was assayed by the induction of thiobarbituric acid reactive species in the skin; immune function was measured by the contact hypersensitivity (CHS) reaction and supported by the changes in epidermal cytokine expression.
Propolis protected significantly and dose-dependently against both sunburn oedema and the suppression of CHS, and (at 100 mg/kg) against lipid peroxidation. The overexpression of IL-10 and the depletion of IL-12 characteristic of photoimmune suppression were markedly reduced by propolis. Further, the upregulation of IL-6 was decreased, and the associated induction of haem oxygenase was shown to play a role in propolis skin protection.
Sydney propolis was able to effectively reduce cutaneous inflammation, immunosuppression and lipid peroxidation induced by UV exposure. It is concluded that Sydney propolis might have strong beneficial protective effects against photodamage and skin cancer development in humans.
蜂胶是一种蜜蜂产品，在传统医学中被用于抗氧化、免疫刺激、抗炎和抗癌作用。在这里，测试了局部应用粗乙醇提取物悉尼蜂胶以防止与光致癌风险增加相关的紫外线辐射诱导损伤的潜力。
在紫外线照射后，将提供 10 至 200 毫克/千克蜂胶的溶液应用于皮肤。通过测量皮肤褶皱厚度的增加来量化暴露于紫外线（290-400nm）引起的炎症；通过在皮肤中诱导硫代巴比妥酸反应性物质来测定脂质过氧化；通过接触超敏反应（CHS）反应来测量免疫功能，并通过表皮细胞因子表达的变化来支持。
蜂胶显著且剂量依赖性地防止了晒伤水肿和 CHS 的抑制，并（在 100 毫克/千克）防止了脂质过氧化。光免疫抑制的特征性 IL-10 过表达和 IL-12 耗竭被蜂胶明显减少。此外，IL-6 的上调减少，并且相关的血红素加氧酶诱导被证明在蜂胶皮肤保护中起作用。
悉尼蜂胶能够有效减少紫外线照射引起的皮肤炎症、免疫抑制和脂质过氧化。因此，可以得出结论，悉尼蜂胶可能对人类的光损伤和皮肤癌发展具有强大的有益保护作用。
