Bioprocess and Molecular Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia,
Cytotechnology. 2010 Oct;62(5):423-30. doi: 10.1007/s10616-010-9294-9. Epub 2010 Oct 16.
This research was conducted to examine the growth profile, growth kinetics, and insulin-secretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex(®) II) and crosslinked polystyrene Plastic Plus (PP) from Solohill Engineering. The cell line producing insulin was cultured in a 25 cm(2) T-flask as control while MCs based culture was implemented in a stirred tank bioreactor with 1 L working volume. For each culture type, the viable cell number, glucose, lactate, glutamate, and insulin concentrations were measured and compared. Maximum viable cell number was obtained at 1.47 × 10(5) cell/mL for PP microcarrier (PPMCs) culture, 1.35 × 10(5) cell/mL Hillex(®) II (HIIMCs) culture and 0.95 × 10(5) cell/mL for T-flask culture, respectively. The highest insulin concentration has been produced in PPMCs culture (5.31 mg/L) compared to HIIMCs culture (2.01 mg/L) and T-flask culture (1.99 mg/L). Therefore overall observation suggested that PPMCs was likely preferred to be used for BRIN-BD11 cell culture as compared with Hillex(®) II MCs.
这项研究旨在考察在优化培养基中生长的 BRIN-BD11 细胞的生长曲线、生长动力学和胰岛素分泌反应性，以及不同类型的微载体（MCs）上的生长情况。我们比较了改性聚苯乙烯（Hillex® II）和交联聚苯乙烯 Plastic Plus（PP）这两种来自 Solohill Engineering 的微载体。胰岛素产生细胞系在 25cm2 的 T 瓶中作为对照进行培养，而基于 MC 的培养则在 1L 工作体积的搅拌槽生物反应器中进行。对于每种培养类型，我们都测量并比较了活细胞数、葡萄糖、乳酸、谷氨酸和胰岛素浓度。结果显示，在 PP 微载体（PPMCs）培养中，最大活细胞数达到 1.47×105 个/mL，在 Hillex® II（HIIMCs）培养中达到 1.35×105 个/mL，在 T 瓶培养中达到 0.95×105 个/mL。在 PPMCs 培养中产生的胰岛素浓度最高（5.31mg/L），其次是 HIIMCs 培养（2.01mg/L）和 T 瓶培养（1.99mg/L）。因此，总体观察表明，与 Hillex® II MCs 相比，PPMCs 更适合用于 BRIN-BD11 细胞培养。
