盐酸多西环素温度敏感性水凝胶对抑制碱性成纤维细胞生长因子诱导大鼠角膜新生血管的作用。
The effect of doxycycline temperature-sensitive hydrogel on inhibiting the corneal neovascularization induced by BFGF in rats.
机构信息
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie South Road, Guangzhou, 510060, China.
出版信息
Graefes Arch Clin Exp Ophthalmol. 2011 Mar;249(3):421-7. doi: 10.1007/s00417-010-1539-y. Epub 2010 Oct 16.
BACKGROUND
To study the effect of doxycycline temperature-sensitive hydrogel (DTSH) on inhibiting the corneal neovascularization (NV) induced by the basic fibroblast growth factor (bFGF).
METHODS
Corneal NV was induced by slow-release polymer pellets containing bFGF, using a rat corneal pocket model. After being implanted with bFGF pellets, the female Sprague-Dawley rats were randomly divided into seven groups (12 rats/group). The grouped rats were given topically normal saline solution and neutralized DTSH at a concentration of 0%, 0.01%, 0.05%, 0.1%, 0.5%, and 1% respectively, and treated for 6 consecutive days. After 6 days of treatment, the cornea was perfused with India ink. The length and area of the corneal vessel were measured and analyzed by Image Pro-Plus 5.1.
RESULTS
Compared to the control group given saline solution, the study groups given DTSH at a concentration of 0.05%, 0.1%, 0.5%, and 1% showed significant reduction in the vessel length (respectively, 58%, 60%, 52%, and 37%) and the vessel area (respectively, 61%, 62%, 49%, and 39%) (p < 0.001). However, no such significant reduction was observed in the study group given 0.01% DTSH (p = 0.133 and 0.166 for vessel length and area respectively). Study groups given 0.05% and 0.1% DTSH showed better effects than groups given 0.01% and 1% DTSH with regard to reducing the vessel length and the vessel area (p < 0.05).
CONCLUSION
The study results showed that topical DTSH effectively inhibited corneal NV at the ideal concentration of 0.05% and 0.1%. Therefore, topical DTSH could be considered as an alternative treatment for the clinical management of corneal NV.
背景
研究盐酸多西环素温敏水凝胶（DTSH）对碱性成纤维细胞生长因子（bFGF）诱导的角膜新生血管（NV）的抑制作用。
方法
使用含 bFGF 的缓释聚合物微球诱导大鼠角膜囊模型中的角膜 NV。在植入 bFGF 微球后，将雌性 Sprague-Dawley 大鼠随机分为 7 组（每组 12 只大鼠）。分组大鼠分别给予局部生理盐水和中和浓度为 0%、0.01%、0.05%、0.1%、0.5%和 1%的 DTSH，连续治疗 6 天。治疗 6 天后，用印度墨水灌流角膜。用 Image Pro-Plus 5.1 测量和分析角膜血管的长度和面积。
结果
与给予生理盐水的对照组相比，给予浓度为 0.05%、0.1%、0.5%和 1%的 DTSH 的研究组，血管长度（分别为 58%、60%、52%和 37%）和血管面积（分别为 61%、62%、49%和 39%）显著降低（p<0.001）。然而，给予 0.01% DTSH 的研究组没有观察到这种显著降低（分别为血管长度和面积的 p=0.133 和 0.166）。与给予 0.01%和 1% DTSH 的研究组相比，给予 0.05%和 0.1% DTSH 的研究组在减少血管长度和血管面积方面效果更好（p<0.05）。
结论
研究结果表明，局部 DTSH 在理想浓度 0.05%和 0.1%时能有效抑制角膜 NV。因此，局部 DTSH 可考虑作为角膜 NV 临床治疗的替代方法。
Zotero 插件