Wu Pei-Chang, Liu Chi-Chang, Chen Chih-hsin, Kou Hsi-Kung, Shen Su-Chin, Lu Cheng-Yuan, Chou Wen-Ying, Sung Ming-Tse, Yang Lin-Cheng
Department of Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung, Taiwan, ROC.
Graefes Arch Clin Exp Ophthalmol. 2003 Jan;241(1):63-9. doi: 10.1007/s00417-002-0591-7. Epub 2002 Dec 18.
This study evaluated the inhibitory activity of somatostatin 14 on the angiogenesis of cornea in vivo.
Corneal neovascularization was induced with a pellet containing 90 ng of basic fibroblast growth factor (bFGF) in a rat corneal pocket model. Three kinds of pellets were made containing bFGF plus somatostatin (SST) 0 ng, 20 ng and 200 ng for the control group, group 1 and group 2, respectively. Neovascularization was observed biomicroscopically from day 4 to day 8, and the corneas were then examined for changes in histology. Quantitation of angiogenesis in the cornea was accomplished by caliper and image analysis.
The 200-ng dose of SST showed significant inhibition of both length and area of neovascularization on day 7 (0.62+/-0.11 mm vs 1.29+/-0.16 mm, 0.50+/-0.16 mm2 vs 1.35+/-0.29mm2, group 2 vs control; P<0.05). The 20 ng of somatostatin did not demonstrate any significant inhibition of neovascularization compared with the control group.
Our study demonstrated that SST 14 can reduce bFGF-induced corneal angiogenesis. This shows the potential value of somatostatin in the treatment of corneal neovascularization.
本研究评估了生长抑素14在体内对角膜血管生成的抑制活性。
在大鼠角膜袋模型中，用含90 ng碱性成纤维细胞生长因子（bFGF）的药粒诱导角膜新生血管形成。分别制备三种药粒，对照组含bFGF加0 ng生长抑素（SST），第1组含bFGF加20 ng SST，第2组含bFGF加200 ng SST。从第4天至第8天用生物显微镜观察新生血管形成情况，然后检查角膜的组织学变化。通过卡尺测量和图像分析对角膜血管生成进行定量。
第7天，200 ng剂量的SST对新生血管的长度和面积均有显著抑制作用（第2组与对照组相比：0.62±0.11 mm对1.29±0.16 mm，0.50±0.16 mm²对1.35±0.29 mm²；P<0.05）。与对照组相比，20 ng生长抑素未显示出对新生血管形成的任何显著抑制作用。
我们的研究表明，SST 14可减少bFGF诱导的角膜血管生成。这显示了生长抑素在治疗角膜新生血管形成方面的潜在价值。
