Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Avian Pathol. 2010 Oct;39(5):359-65. doi: 10.1080/03079457.2010.506211.
To investigate the host-pathogen interactions between infectious bursal disease virus (IBDV) and target B-lymphocytic cells, a cDNA T7 phage display library from the chicken bursa of Fabricius was constructed and screened for virus binding. Surface immunoglobulin M (sIgM) was isolated as a putative candidate binding site and its interactions with IBDV were further investigated using a chicken bursal lymphoma-derived cell line DT40. The results showed that the λ light chain of sIgM specifically interacted with IBDV in a virulence-independent manner in vitro, and most of the binding of IBDV to DT40 cells was inhibited by sIgM-specific monoclonal antibodies. Further, the infectivity of IBDV in vitro was reduced by sIgM-specific monoclonal antibodies. Our data provided evidence that sIgM may participate as one of the putative membrane binding sites responsible for IBDV infection.
为了研究传染性法氏囊病病毒（IBDV）与靶 B 淋巴细胞之间的宿主-病原体相互作用，构建了鸡法氏囊的 cDNA T7 噬菌体展示文库，并进行了病毒结合筛选。表面免疫球蛋白 M（sIgM）被分离为一个假定的候选结合位点，并使用鸡法氏囊淋巴瘤衍生的细胞系 DT40 进一步研究了其与 IBDV 的相互作用。结果表明，sIgM 的 λ 轻链在体外以与毒力无关的方式特异性地与 IBDV 相互作用，并且 sIgM 特异性单克隆抗体抑制了 IBDV 与 DT40 细胞的大部分结合。此外，sIgM 特异性单克隆抗体降低了 IBDV 的体外感染性。我们的数据提供了证据表明，sIgM 可能作为负责 IBDV 感染的假定膜结合位点之一参与其中。
