Medical Clinic 1, Friedrich-Alexander-University, Erlangen, Germany.
Gastroenterology. 2010 Dec;139(6):1837-1843.e1. doi: 10.1053/j.gastro.2010.10.007. Epub 2010 Oct 16.
Mouse models of intestinal inflammation and colon cancer are valuable tools to gain insights into the pathogenesis of the corresponding human diseases. Recently, in vivo mouse endoscopy has been developed, allowing not only the high-resolution monitoring and scoring of experimental disease development, but also enables the investigator to perform manipulations, including local injection of reagents or the taking of biopsies for molecular and histopathologic analyses. Chromoendoscopic staining with methylene blue enables visualization of the crypt structure and allows discrimination between inflammatory and neoplastic changes. The development of endoscopic techniques in live mice opened new options for the investigation of disease mechanisms in the gut and for the preclinical testing of potential therapeutic effects of drug candidates. Finally, mouse endoscopy can help to reduce animal numbers needed to gain significant experimental data.
肠道炎症和结肠癌的小鼠模型是深入了解相应人类疾病发病机制的重要工具。最近，活体小鼠内镜技术得以发展，不仅可以高分辨率地监测和评分实验性疾病的进展，还允许研究者进行操作，包括局部注射试剂或进行活检以进行分子和组织病理学分析。亚甲蓝染色内镜检查可使隐窝结构可视化，并有助于区分炎症和肿瘤性变化。活体小鼠内镜技术的发展为肠道疾病机制的研究以及候选药物的临床前疗效测试提供了新的选择。最后，小鼠内镜检查有助于减少获得有意义的实验数据所需的动物数量。
