CIEPQPF, Departamento de Engenharia Química, Universidade de Coimbra, Polo II, Pinhal de Marrocos, 3030-290 Coimbra, Portugal.
Int J Biol Macromol. 2011 Jan 1;48(1):112-8. doi: 10.1016/j.ijbiomac.2010.10.006. Epub 2010 Oct 16.
In this work, porous scaffolds obtained from the freeze-drying of pectin/chitosan polyelectrolyte complexes were prepared and characterized by FTIR, SEM and weight loss studies. Additionally, the cytotoxicity of the prepared scaffolds was evaluated in vitro, using human osteoblast cells. The results obtained showed that cells adhered to scaffolds and proliferated. The study also confirmed that the degradation by-products of pectin/chitosan scaffold are noncytotoxic.
本工作通过冻融干燥法制备了果胶/壳聚糖聚电解质复合物多孔支架，并通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和重量损失研究对其进行了表征。此外，还通过体外培养人成骨细胞评估了所制备支架的细胞毒性。结果表明，细胞黏附在支架上并增殖。研究还证实，果胶/壳聚糖支架的降解产物无细胞毒性。
