da Paz Leôncio Alves Simone, Jacob Iris Trindade Tenório, Arruda Marcela Daniela Muniz, da Silva Abdênego Rodrigues, de Sousa Georon Ferreira, de Souza Guilherme Antônio, de Lima Maria do Carmo Alves, de Souza Ivone Antônia, de Melo Cristiane Moutinho Lagos, da Cruz Filho Iranildo José, do Nascimento Santos Dayane Kelly Dias
Department of Antibiotics, Biosciences Center, Federal University of Pernambuco, Recife, PE 50.670-420 Brazil.
Department of Biochemistry, Biosciences Center, Federal University of Pernambuco, Recife, PE 50.670-420 Brazil.
3 Biotech. 2023 Apr;13(4):114. doi: 10.1007/s13205-023-03509-y. Epub 2023 Mar 9.
The objective of this work was to isolate a polysaccharide similar to pectin from leaves, not yet reported in the literature, and to evaluate its antioxidant, cytotoxic and immunomodulatory profile. Pectin was extracted from the leaves in three stages, organic solvent followed by acidified water and ethanol precipitation. With the pectin obtained, the physicochemical characterization of the molecule was carried out using high-performance liquid chromatography, Fourier-transform infrared spectroscopy, nuclear magnetic resonance (C and H) and different thermal and elemental analysis. Furthermore, the antioxidant activities were evaluated in vitro, and using human peripheral blood mononuclear cell culture, cytotoxicity and immunostimulatory actions were investigated. Physical and chemical analyses showed characteristic signs of pectin. Antioxidant activity tests showed that pectin had moderate to low antioxidant activity. Furthermore, pectin did not affect the viability of erythrocytes and PBMC and induced an immunostimulatory state when it promoted the production of cytokines IL-6, IL-10 and TNF-α and increased the activation of CD8 + T lymphocytes. This study showed that pectin from is not cytotoxic and promoted a pro-inflammatory profile in peripheral blood mononuclear cell with application as an immunostimulating and emulsifying compound.
本研究的目的是从叶片中分离出一种尚未见文献报道的类似果胶的多糖，并评估其抗氧化、细胞毒性和免疫调节特性。果胶从叶片中分三个阶段提取，先用有机溶剂，接着用酸化水，最后乙醇沉淀。利用得到的果胶，采用高效液相色谱、傅里叶变换红外光谱、核磁共振（碳谱和氢谱）以及不同的热分析和元素分析对该分子进行了理化特性表征。此外，还进行了体外抗氧化活性评估，并利用人外周血单核细胞培养研究了细胞毒性和免疫刺激作用。理化分析显示出果胶的特征性迹象。抗氧化活性测试表明，果胶具有中度至低度的抗氧化活性。此外，果胶不影响红细胞和外周血单核细胞的活力，并且在促进细胞因子IL-6、IL-10和TNF-α的产生以及增加CD8 + T淋巴细胞的活化时，诱导了一种免疫刺激状态。本研究表明，[植物名称]的果胶无细胞毒性，并在外周血单核细胞中促进了促炎特性，可作为免疫刺激和乳化化合物应用。
