Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India.
Food Chem Toxicol. 2011 Jan;49(1):173-8. doi: 10.1016/j.fct.2010.10.013. Epub 2010 Oct 16.
The modifying effect of chemically modified chrysin on formation of preneoplastic foci induced by N-nitrosodiethylamine (DEN) was investigated in male rats. Male Wistar rats were administered three intraperitoneal injections of DEN (200 mg/kg bodyweight) interspersed by 2 weeks with or without an oral dose of dimethoxy flavone (DMF 100 mg/kg bodyweight), 2 weeks after DEN initiation. The number of GST-Pi positive foci and proliferating cell nuclear antigen (PCNA)-positive cells were significantly suppressed by the administration of DMF. Real-time RT-PCR analysis revealed that DMF treatment increased mRNA expression levels of apoptotic proteins p53 and fas, cell cycle regulatory proteins chek 2, cdkn1a, rad 50, anti-inflammatory protein pparg whereas the mRNA expression levels of bcl-2 and prdx-2 were decreased compared to mRNA levels in DEN-treated group. Therefore, we propose that DMF partially suppresses the formation of preneoplastic lesions in rats following DEN exposure by regulating anti-inflammatory and apoptosis-promoting events and restoring the cellular redox balance altered by DEN.
研究了化学修饰的白杨素对 N-亚硝基二乙胺（DEN）诱导的雄性大鼠前癌灶形成的修饰作用。雄性 Wistar 大鼠给予 DEN（200mg/kg 体重）三次腹腔注射，间隔 2 周，并用或不用二甲氧基黄酮（DMF 100mg/kg 体重）口服，DEN 起始后 2 周。GST-Pi 阳性病灶和增殖细胞核抗原（PCNA）阳性细胞的数量明显被 DMF 给药抑制。实时 RT-PCR 分析显示，DMF 处理增加了促凋亡蛋白 p53 和 fas、细胞周期调节蛋白 chek2、cdkn1a、rad50、抗炎蛋白 pparg 的 mRNA 表达水平，而 bcl-2 和 prdx-2 的 mRNA 表达水平与 DEN 处理组相比则降低。因此，我们提出 DMF 通过调节抗炎和促进凋亡事件以及恢复 DEN 改变的细胞氧化还原平衡，部分抑制 DEN 暴露后大鼠前癌病变的形成。
