Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu-shi, Tokyo 183-8509, Japan.
Toxicology. 2010 Feb 9;268(3):213-8. doi: 10.1016/j.tox.2009.12.019. Epub 2010 Jan 5.
To investigate the modifying effect of enzymatically modified isoquercitrin (EMIQ) on hepatocellular tumor promotion induced by beta-naphthoflavone (BNF) treatment, male rats were administered a single intraperitoneal injection of N-diethylnitrosamine (DEN) and were fed a diet containing BNF (0.5%) for 6 weeks with or without EMIQ (0.2%) in the drinking water after DEN initiation. One week after the commencement of the administration of BNF, rats were subjected to a two-thirds partial hepatectomy. The number and area of GST-P positive foci, the number of COX2-positive cells and the area of elastica-van Gieson (EVG)-positive connective tissue fibers promoted by BNF were significantly suppressed by the administration of the antioxidant EMIQ. Real-time RT-PCR analysis revealed that EMIQ treatment decreased mRNA expression levels of Gstm1, Serpine1, Cox2 and Nfkbia and increased mRNA expression levels of Yc2 compared with those in the DEN-BNF group. These results suggest that co-administration of EMIQ suppresses the hepatocellular tumor-promoting activity of BNF in rats through the anti-inflammatory effects of EMIQ and restores the cellular redox balance altered by BNF.
为了研究酶法改性异槲皮苷（EMIQ）对β-萘黄酮（BNF）诱导的肝细胞肿瘤促进作用的修饰作用，雄性大鼠一次性腹腔注射 N-二乙基亚硝胺（DEN），并在 DEN 诱导后用含有 BNF（0.5%）的饮食喂养 6 周，同时在饮水中添加或不添加 EMIQ（0.2%）。在开始给予 BNF 的一周后，大鼠接受三分之二的部分肝切除术。BNF 诱导的 GST-P 阳性焦点的数量和面积、COX2 阳性细胞的数量以及弹性蛋白-van Gieson（EVG）阳性结缔组织纤维的面积均被抗氧化剂 EMIQ 的给药显著抑制。实时 RT-PCR 分析显示，与 DEN-BNF 组相比，EMIQ 处理降低了 Gstm1、Serpine1、Cox2 和 Nfkbia 的 mRNA 表达水平，同时增加了 Yc2 的 mRNA 表达水平。这些结果表明，EMIQ 的共同给药通过 EMIQ 的抗炎作用抑制了 BNF 在大鼠中的肝细胞肿瘤促进活性，并恢复了 BNF 改变的细胞氧化还原平衡。
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