Department of Immunology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan.
J Exp Med. 2010 Oct 25;207(11):2493-506. doi: 10.1084/jem.20100760. Epub 2010 Oct 18.
Polycomb group (PcG) and trithorax group (TrxG) complexes exert opposing effects on the maintenance of the transcriptional status of the developmentally regulated Hox genes. In this study, we show that activation of STAT6 induces displacement of the PcG complex by the TrxG complex at the upstream region of the gene encoding GATA3, a transcription factor essential for T helper type 2 (Th2) cell differentiation. Once Th2 cells differentiate, TrxG complex associated with the TrxG component Menin binds to the whole GATA3 gene locus, and this binding is required for the long-term maintenance of expression of GATA3 and Th2 cytokine. Thus, STAT6-mediated displacement of PcG by the TrxG complex establishes subsequent STAT6-independent maintenance of GATA3 expression in Th2 cells via the recruitment of the Menin-TrxG complex.
多梳抑制复合物（PcG）和三进制组（TrxG）复合物对发育调节的 Hox 基因的转录状态的维持具有相反的作用。在这项研究中，我们表明，STAT6 的激活诱导 PcG 复合物被 TrxG 复合物在编码 GATA3 的基因的上游区域置换，GATA3 是 T 辅助细胞 2 型（Th2）细胞分化所必需的转录因子。一旦 Th2 细胞分化，与 TrxG 成分 Menin 相关的 TrxG 复合物结合到整个 GATA3 基因座，并且这种结合对于 GATA3 和 Th2 细胞因子表达的长期维持是必需的。因此，STAT6 介导的 PcG 由 TrxG 复合物置换在 Th2 细胞中通过募集 Menin-TrxG 复合物建立随后的 STAT6 独立的 GATA3 表达的维持。
