Nutrición y Bromatología, Escuela de Ingenieńas Agrarias, University ofExtremadura, Ctra. de Cáceres s/n, 06071 Badajoz, Spain.
J Agric Food Chem. 2010 Nov 24;58(22):11688-94. doi: 10.1021/jf102414q. Epub 2010 Oct 19.
The purpose of this work was to develop a PCR method for the identification of smoked paprika "Pimentón de la Vera" adulteration with paprika elaborated from varieties of pepper foreign to the la Vera region, in central western Spain. Three autochthonous varieties of pepper, Jaranda, Jariza, and Bola, and the varieties Papri Queen, Papri King, Sonora, PS9794, and Papri Ace, foreign to the La Vera region, were used in the study. Analyses of the ITS and 5.8S rDNA, RAPD-PCR, SSR, and ISSR were tested. RAPD-PCR, SSR, and ISSR analyses allowed differentiation among the varieties of paprika analyzed. There was no difference in the sequence of ITS1-5.8S rDNA-ITS2. In addition, with the RAPD-PCR primers S13 and S22, two molecular markers were obtained of 641 and 704 bp, respectively, which allowed all of the smoked paprika varieties to be differentiated from paprikas elaborated with the five foreign varieties. These two molecular markers were investigated as a basis for detecting the adulteration of smoked paprika with paprika elaborated from foreign varieties of pepper.
本研究旨在开发一种 PCR 方法，以鉴定来自西班牙中西部拉韦拉地区以外品种的辣椒粉对熏辣椒粉“Pimentón de la Vera”的掺假情况。Jaranda、Jariza 和 Bola 三种本土辣椒品种以及 Papri Queen、Papri King、Sonora、PS9794 和 Papri Ace 五种外来品种均用于本研究。对 ITS 和 5.8S rDNA、RAPD-PCR、SSR 和 ISSR 进行了分析。RAPD-PCR、SSR 和 ISSR 分析可区分所分析的辣椒粉品种。ITS1-5.8S rDNA-ITS2 序列无差异。此外，使用 RAPD-PCR 引物 S13 和 S22，分别获得了 641bp 和 704bp 的两个分子标记，可将所有熏辣椒粉品种与用五种外来品种制成的辣椒粉区分开来。这两个分子标记可作为检测熏辣椒粉中掺假外来品种辣椒粉的基础。
