Gallardo G, Ruiz-Moyano S, Hernández A, Benito M J, Córdoba M G, Pérez-Nevado F, Martín A
Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Av. Adolfo Suarez, 06007 Badajoz, Spain.
Food Microbiol. 2014 Sep;42:205-11. doi: 10.1016/j.fm.2014.03.022. Epub 2014 Apr 4.
Yeast populations of dry-cured Iberian ham isolated from seven industries in the province of Badajoz were characterized by ISSR-PCR using the (CAG)4 primer and PCR-RFLP of the ITS1-5.8S rRNA-ITS2 fragment, and identified by DNA sequencing. A total of 242 isolates were analyzed, indicating the primary species present was Debaryomyces hansenii at 80.9% of the isolates followed by Candida zeylanoides at 10.3% of the isolates. The remainders of isolates were identified as Yamadazyma triangularis, Sporobolomyces roseus, Meyerozyma guilliermondii, Rhodotorula slooffiae, and Cryptococcus victoriae. The ISSR-PCR method was a fast and reliable method which was able to discriminate species at a level comparable to restriction analyses of the ITS1-5.8S rRNA-ITS2 region. This method allowed for strain typing of D. hansenii, yielding 29 different PCR patterns within 196 isolates. Moreover, ISSR-PCR using the (CAG)4 primer indicated that this technique could be a promising tool for rapid discrimination of yeast starter cultures and spoilage species in dry-cured Iberian ham.
利用（CAG）4引物通过ISSR-PCR以及ITS1-5.8S rRNA-ITS2片段的PCR-RFLP对从巴达霍斯省七个行业分离出的伊比利亚干腌火腿酵母菌群进行了表征，并通过DNA测序进行了鉴定。共分析了242个分离株，结果表明主要存在的菌种是汉逊德巴利酵母，占分离株的80.9%，其次是锡兰假丝酵母，占分离株的10.3%。其余分离株被鉴定为三角山马兹酵母、玫瑰掷孢酵母、季也蒙毕赤酵母、斯洛菲红酵母和维多利亚隐球菌。ISSR-PCR方法是一种快速可靠的方法，能够在与ITS1-5.8S rRNA-ITS2区域的限制性分析相当的水平上区分菌种。该方法可对汉逊德巴利酵母进行菌株分型，在196个分离株中产生了29种不同的PCR图谱。此外，使用（CAG）4引物的ISSR-PCR表明，该技术可能是快速区分伊比利亚干腌火腿中酵母发酵剂培养物和腐败菌种的一种有前景的工具。
