Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
Vaccine. 2010 Dec 6;28(52):8288-99. doi: 10.1016/j.vaccine.2010.10.006. Epub 2010 Oct 16.
Ovalbumin (OVA) was labeled with a near infra-red dye (*OVA) and formulated with the host defense peptide indolicidin (Indol), CpG oligodeoxynucleotide (ODN) 1826 (CpG) and/or poly(p-dicarboxylatophenoxy)-phosphazene (PP4). The immunogenicity of these *OVA formulations was evaluated in mice. All double and triple adjuvant combinations elicited strong antibody responses. *OVA formulated with CpG ODN in combination with indolicidin, PP4 or both induced only IFN-γ, while formulations with indolicidin and/or PP4 promoted predominantly IL-5 production. Overall, both IgG and IFN-γ production was superior when *OVA was combined with CpG/Indol/PP4. Furthermore, mice injected with *OVA formulated with CpG/Indol/PP4 contained detectable *OVA in the injection site two months post immunization. These results indicate that the CpG/Indol/PP4 combination promotes prolonged antigen retention and strong, antigen-specific Th1-biased immune responses.
卵清蛋白 (OVA) 被一种近红外染料标记 (*OVA)，并与宿主防御肽抑菌肽 (Indol)、CpG 寡脱氧核苷酸 (ODN) 1826 (CpG) 和/或聚 (对二羧基苯氧基) - 磷腈 (PP4) 联合制成制剂。这些 *OVA 制剂的免疫原性在小鼠中进行了评估。所有双佐剂和三佐剂组合都引起了强烈的抗体反应。*OVA 与 CpG ODN 联合使用佐剂与抑菌肽、PP4 或两者联合仅诱导 IFN-γ，而与抑菌肽和/或 PP4 联合使用的制剂主要促进 IL-5 的产生。总的来说，当 *OVA 与 CpG/Indol/PP4 联合使用时，IgG 和 IFN-γ 的产生都更好。此外，在免疫后两个月，注射了用 CpG/Indol/PP4 制成的 *OVA 制剂的小鼠在注射部位可检测到 *OVA。这些结果表明，CpG/Indol/PP4 联合促进了抗原的长期保留和强烈的、抗原特异性的 Th1 偏向免疫反应。
