Institute of Physiology II, University of Freiburg, and Centre for Biological Signalling Studies (Bioss),Freiburg, Germany.
Physiol Rev. 2010 Oct;90(4):1437-59. doi: 10.1152/physrev.00049.2009.
Molecular research on ion channels has demonstrated that many of these integral membrane proteins associate with partner proteins, often versatile in their function, or even assemble into stable macromolecular complexes that ensure specificity and proper rate of the channel-mediated signal transduction. Calcium-activated potassium (K(Ca)) channels that link excitability and intracellular calcium concentration are responsible for a wide variety of cellular processes ranging from regulation of smooth muscle tone to modulation of neurotransmission and control of neuronal firing pattern. Most of these functions are brought about by interaction of the channels' pore-forming subunits with distinct partner proteins. In this review we summarize recent insights into protein complexes associated with K(Ca) channels as revealed by proteomic research and discuss the results available on structure and function of these complexes and on the underlying protein-protein interactions. Finally, the results are related to their significance for the function of K(Ca) channels under cellular conditions.
分子研究表明，离子通道的许多整合膜蛋白与功能多样的伴侣蛋白相互作用，甚至组装成稳定的大分子复合物，从而确保通道介导的信号转导的特异性和适当速率。钙激活钾 (K(Ca)) 通道将兴奋性和细胞内钙离子浓度联系起来，负责多种细胞过程，从调节平滑肌张力到调节神经递质传递和控制神经元放电模式。这些功能大多是通过通道的孔形成亚基与独特的伴侣蛋白相互作用实现的。在这篇综述中，我们总结了蛋白质组学研究揭示的与 K(Ca) 通道相关的蛋白复合物的最新见解，并讨论了这些复合物的结构和功能以及潜在的蛋白-蛋白相互作用的研究结果。最后，将这些结果与它们在细胞条件下对 K(Ca) 通道功能的意义联系起来。
