使用希森美康 XE-5000 进行自动脑脊液细胞计数：是否需要新的参考范围？

Automated cerebrospinal fluid cell counts using the Sysmex XE-5000: is it time for new reference ranges?

机构信息

Dept of Pathology, University Hospitals Case Medical Center, 11100 Euclid Ave, Cleveland, OH 44106, USA.

出版信息

Am J Clin Pathol. 2010 Nov;134(5):734-8. doi: 10.1309/AJCPABGQXSIA4SMT.

PMID:20959656

Abstract

The main objectives of the study were to compare manual and automated WBC counts on clear cerebrospinal fluid (CSF) samples. Clear CSF samples from 200 adults and children were studied. Cell counts were performed manually using a hemocytometer and then analyzed on the Sysmex XE-5000. Descriptive statistics and Spearman correlation for nonparametric data were used for method comparison. Manual WBC counts ranged from 0 to 702 cells/μL, and Sysmex counts ranged from 0 to 629 cells/μL. The Spearman rank correlation coefficient for the entire range of data was 0.77 (P < .001); however, the correlation was weaker at the low end of the data spectrum. For manual WBC ranges of 0 to 5 cells/μL and 0 to 10 cells/μL, the corresponding Sysmex 0 to 95th percentile ranges were 0 to 23 cells/μL and 0 to 27 cells/μL, respectively. The results suggest that larger studies are necessary to determine new reference ranges for automated CSF WBC counts.

摘要

本研究的主要目的是比较脑脊液（CSF）样本的手动和自动白细胞计数。研究了 200 例成人和儿童的清亮 CSF 样本。使用血球计数器手动进行细胞计数，然后在 Sysmex XE-5000 上进行分析。使用非参数数据的描述性统计和斯皮尔曼相关性进行方法比较。手动 WBC 计数范围为 0 至 702 个/μL，Sysmex 计数范围为 0 至 629 个/μL。整个数据范围的斯皮尔曼等级相关系数为 0.77（P<.001）；然而，在数据谱的低端相关性较弱。对于手动 WBC 范围为 0 至 5 个/μL 和 0 至 10 个/μL，相应的 Sysmex 0 至 95 百分位范围分别为 0 至 23 个/μL 和 0 至 27 个/μL。结果表明，需要进行更大的研究来确定自动化 CSF WBC 计数的新参考范围。