Department of Chemistry, Emory University, Atlanta, GA 30322, USA.
Chem Commun (Camb). 2010 Dec 14;46(46):8803-5. doi: 10.1039/c0cc02961k. Epub 2010 Oct 19.
We report the computational enzyme design of an orthogonal nucleoside analog kinase for 3'-deoxythymidine. The best kinase variant shows an 8500-fold change in substrate specificity, resulting from a 4.6-fold gain in catalytic efficiency for the nucleoside analog and a 2000-fold decline for the native substrate thymidine.
我们报告了一种用于 3'-脱氧胸苷的正交核苷类似物激酶的计算酶设计。最佳激酶变体显示出 8500 倍的底物特异性变化，这是由于核苷类似物的催化效率提高了 4.6 倍，而天然底物胸苷的活性降低了 2000 倍。
