Qiu L S, Xue H C, Lu J, Zhang Y H, Zhu Z X, Li H
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(3):177-80.
In the present paper, the results of detecting circulating antigen and/or antigen-antibody complexes by McAb against surface membrane antigen of adult Schistosoma japonicum were reported. The McAb, coded as 8SE4, was prepared by fusion of SP2/0 myeloma cells with spleen cells of the BALB/c mice immunized with the saline extract of adult S. japonicum. The 8SE4- directed antigen was proved to be located on the surface of the adult worm. After being purified by a DE52 column, 8SE4 was labelled with HRP and the conjugate (HRP-8SE4) was used in the test. For testing, the serum sample was first incubated with HRP-8SE4, then PEG (mw. 6,000) was added to precipitate the antigen-antibody complex. Upon centrifugation, OPD was added to the precipitate. Results were read by ELISA reader at 492nm. The OD value was found to be proportional to the amount of circulating antigen and/or antigen-antibody complexes. Results from 5 heavily infected (1,500-2,000 cercariae) rabbits showed that the OD values were raised significantly at the 6th week post infection, being 1.9-4.5 times higher than those before infection. The OD values of the 5 rabbits each lightly infected with 10-500 cercariae were also markedly raised 6 weeks post infection and reached the peak at the 8th week, then maintained in high levels until 11th week post infection. The worm burden of the 5 lightly infected rabbits were 4-326. No obvious correlations between OD values and worm loads were observed. The results suggested the existence of surface membrane-related antigen and/or antigen-antibody complexes in the circulation of infected rabbits.
本文报道了用抗日本血吸虫成虫表面膜抗原的单克隆抗体检测循环抗原和/或抗原抗体复合物的结果。编码为8SE4的单克隆抗体是通过将SP2/0骨髓瘤细胞与用日本血吸虫成虫盐水提取物免疫的BALB/c小鼠的脾细胞融合制备的。已证明8SE4所针对的抗原位于成虫表面。经DE52柱纯化后，8SE4用辣根过氧化物酶（HRP）标记，标记物（HRP-8SE4）用于检测。检测时，血清样本先与HRP-8SE4孵育，然后加入聚乙二醇（分子量6000）沉淀抗原抗体复合物。离心后，向沉淀物中加入邻苯二胺（OPD）。用酶标仪在492nm处读取结果。发现光密度（OD）值与循环抗原和/或抗原抗体复合物的量成正比。5只重度感染（1500 - 2000尾尾蚴）兔的结果显示，感染后第6周OD值显著升高，比感染前高1.9 - 4.5倍。5只轻度感染10 - 500尾尾蚴的兔在感染后6周OD值也明显升高，并在第8周达到峰值，然后一直维持在高水平直至感染后第11周。5只轻度感染兔的虫荷为4 - 326条。未观察到OD值与虫荷之间有明显相关性。结果提示在感染兔的循环中存在表面膜相关抗原和/或抗原抗体复合物。
