Qiu L, Zhang Y, Chen W, Li H, Xue H
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(3):221-2.
Single sex cercariae were used to infect rabbits and the detection of circulating membrane antigen by dot-ELISA were followed up until 1 year postinfection. 9 rabbits infected with male cercariae (worm recovery 15-133, mean 53.1) and 9 rabbits infected with female cercariae (worm recovery 1-102, mean 39.1) were examined but no positive reaction was occurred. On the contrary, 7 rabbits infected with bi-sex cercariae (worm recovery 4-161, mean 67.1) all showed positive reactions 6-10 wks post-infection. Uni-sex female worms usually could not develop to maturity within rabbits even 1 year after infection, suggesting that the life-span of uni-sex female worm might be 1 year at least in rabbits. The uni-sex male worms could develop to maturity, being usually stout. The question why no circulating membrane antigen (even CCA, unpublished data) could be detected and the nature of antigen we detected needs further investigation.
使用单性尾蚴感染兔子，并通过斑点酶联免疫吸附测定法（dot-ELISA）对循环膜抗原进行检测，持续追踪至感染后1年。对9只感染雄性尾蚴的兔子（虫体回收率为15 - 133，平均为53.1）和9只感染雌性尾蚴的兔子（虫体回收率为1 - 102，平均为39.1）进行了检查，但未出现阳性反应。相反，7只感染两性尾蚴的兔子（虫体回收率为4 - 161，平均为67.1）在感染后6 - 10周均呈现阳性反应。单性雌性虫体即使在感染后1年通常也无法在兔子体内发育成熟，这表明单性雌性虫体在兔子体内的寿命可能至少为1年。单性雄性虫体能够发育成熟，通常较为粗壮。为何检测不到循环膜抗原（甚至是CCA，未发表数据）以及我们所检测到的抗原的性质，需要进一步研究。
