Wu H, Li Y H
Department of Parasitology, Suzhou Medical College.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(3):210-3.
The indirect immunofluorescent antibody test (IFAT) and immunoenzymatic staining technique (IEST) with frozen sections of Brugia malayi and Setaria cervi adult worms as antigens were used to detect IgG levels in rabbits immunized with three different filarial antigens. The results showed the dynamics of the specific IgG antibody in the course of immunization. The peaks (GMRT 76.11-861.08) of IgG levels in immunized rabbits appeared at the 4th week after primary immunization, which returned to (4.00-32.0 up to the 8th week after primary immunization and rose to (20.11-181.02) at the 1st week after second immunization. It is suggested that IFAT and IEST, especially the latter, can be applied for serodiagnosis of filarial infection and both antigens are considered to be of similar diagnostic effect.
以马来布鲁线虫和猪鬃尾线虫成虫冰冻切片为抗原，采用间接免疫荧光抗体试验（IFAT）和免疫酶染色技术（IEST）检测用三种不同丝虫抗原免疫的家兔体内IgG水平。结果显示了免疫过程中特异性IgG抗体的动态变化。免疫家兔IgG水平的峰值（几何平均滴度76.11 - 861.08）出现在初次免疫后的第4周，在初次免疫后的第8周降至（4.00 - 32.0），并在第二次免疫后的第1周升至（20.11 - 181.02）。提示IFAT和IEST，尤其是后者，可用于丝虫感染的血清学诊断，且两种抗原的诊断效果相似。
