[间接血凝试验（IEST）和间接荧光抗体试验（IFAT）诊断华支睾吸虫病的比较研究]

[Comparative studies on the diagnosis of clonorchiasis by IEST and IFAT].

作者信息

Liu X M, Hu W F, Wang Z H, Chen D L, Xu F N

机构信息

Anhui Provincial Institute of Parasitic Diseases, Wuhu.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(4):273-5.

PMID:7521804

Abstract

Both immunoenzymatic staining technique (IEST) and indirect fluorescent antibody test. (IFAT) with frozen sections of adult Clonorchis sinensis as antigen were employed for detecting 51 cases with clonorchiasis and 50 healthy persons. The positive rate was 92% with IEST and 88% with IFAT. The results showed no significant statistic difference (P > 0.05). The false positive rates were 2% with the former and 4% with the latter. When sera from 22 cases with acute schistosomiasis, 20 cases with chronic schistosomiasis and 15 cases with paragonimiasis were examined by IEST and IFAT, cross-reactions were 14%, 5% and 0% with IEST, and 14%, 10% and 0% with IFAT, respectively. The results showed that both IEST and IFAT are useful methods for serological diagnosis of clonorchiasis and the antigen on the gut was well demonstrated, while IEST might be more suitable in field surveys.

摘要

以华支睾吸虫成虫冰冻切片为抗原，采用免疫酶染色技术（IEST）和间接荧光抗体试验（IFAT）对51例华支睾吸虫病患者和50名健康人进行检测。IEST阳性率为92%，IFAT阳性率为88%。结果显示差异无统计学意义（P>0.05）。前者假阳性率为2%，后者为4%。用IEST和IFAT检测22例急性血吸虫病患者、20例慢性血吸虫病患者和15例肺吸虫病患者的血清，IEST的交叉反应率分别为14%、5%和0%，IFAT的交叉反应率分别为14%、10%和0%。结果表明，IEST和IFAT都是华支睾吸虫病血清学诊断的有用方法，肠道抗原显示良好，而IEST可能更适合现场调查。