核酮糖-1,5-二磷酸羧化酶/加氧酶激活酶活性测定

Rubisco activase activity assays.

作者信息

Barta Csengele, Carmo-Silva A Elizabete, Salvucci Michael E

机构信息

U.S. Department of Agriculture, Arid-Land Agricultural Research Center, Maricopa, AZ, USA.

出版信息

Methods Mol Biol. 2011;684:375-82. doi: 10.1007/978-1-60761-925-3_29.

DOI:10.1007/978-1-60761-925-3_29

PMID:20960144

Abstract

Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites, thereby restoring the sites to catalytic competence. This chapter reports methods developed for assaying the two activities of Rubisco activase: ATP hydrolysis and Rubisco activation.

摘要

1,5-二磷酸核酮糖羧化酶/加氧酶（Rubisco）活化酶作为一种机械化学运动蛋白，利用ATP水解产生的能量使靶蛋白Rubisco的结构发生扭曲。这一作用通过从Rubisco的催化位点去除紧密结合的抑制性糖磷酸来调节Rubisco的活化状态，从而使这些位点恢复催化活性。本章介绍了用于测定Rubisco活化酶两种活性的方法：ATP水解和Rubisco活化。