Department of Biology of Shantou University, Shantou, 515063, China.
Virol Sin. 2010 Oct;25(5):341-51. doi: 10.1007/s12250-010-3137-x. Epub 2010 Oct 8.
In this paper, in vitro anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis were investigated. Cytotoxicities and antiviral activities of Gracilaria lemaneiformis polysaccharides (PGL), Gracilaria lemaneiformis polysaccharide fraction-1 (GL-1), Gracilaria lemaneiformis polysaccharide fraction-2 (GL-2) and Gracilaria lemaneiformis polysaccharide fraction-3 (GL-3) were studied by the Methyl thiazolyl tetrazolium (MTT) method, and the inhibitory effect against Human influenza virus H1-364 induced cytopathic effect (CPE) on MDCK cells were observed by the CPE method. In addition, the antiviral mechanism of PGL was explored by Plaque forming unit (PFU), MTT and CPE methods. The results showed: i) Cytotoxicities were not significantly revealed, and H1-364 induced CPE was also reduced treated with sulfated polysaccharide fractions from Gracilaria lemaneiformis; ii) Antiviral activities were associated with the mass percentage content of sulfate groups in polysaccharide fractions, which was about 13%, in polysaccharides (PGL and GL-2) both of which exhibited higher antiviral activity; iii) A potential antiviral mechanism to explain these observations is that viral adsorption and replication on host cells were inhibited by sulfated polysaccharides from Gracilaria lemaneiformis. In conclusion, Anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis were revealed, and the antiviral activities were associated with content of sulfate groups in polysaccharide fractions.
本文研究了龙须菜硫酸多糖组分的体外抗流感病毒活性。采用 MTT 法研究了龙须菜多糖（PGL）、龙须菜多糖组分 1（GL-1）、龙须菜多糖组分 2（GL-2）和龙须菜多糖组分 3（GL-3）的细胞毒性和抗病毒活性，采用细胞病变效应（CPE）法观察了它们对 MDCK 细胞感染人流感病毒 H1-364 的抑制作用。此外，还通过空斑形成单位（PFU）、MTT 和 CPE 法探讨了 PGL 的抗病毒机制。结果表明：i）硫酸多糖组分对细胞无明显毒性，且能减轻 H1-364 诱导的细胞病变效应；ii）抗病毒活性与多糖组分中硫酸基团的质量百分比含量有关，多糖（PGL 和 GL-2）的硫酸基团质量百分比含量约为 13%，显示出较高的抗病毒活性；iii）一种可能的抗病毒机制解释了这些观察结果，即硫酸多糖抑制了病毒在宿主细胞上的吸附和复制。综上所述，龙须菜硫酸多糖组分具有抗流感病毒活性，其抗病毒活性与多糖组分中硫酸基团的含量有关。
