传染性胃肠炎病毒 TS 的全基因组序列和细胞培养后的 3'末端序列特征
Complete genomic sequence of transmissible gastroenteritis virus TS and 3' end sequence characterization following cell culture.
机构信息
Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
出版信息
Virol Sin. 2010 Jun;25(3):213-24. doi: 10.1007/s12250-010-3108-2. Epub 2010 Jun 6.
Abstract
The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5' non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.
摘要
传染性胃肠炎病毒（TGEV）株 TS 的全基因组序列先前从甘肃省分离得到，现已被克隆并与其他 TGEV 株的已发表序列数据进行了比较。基于 S 基因的氨基酸和核苷酸序列构建的系统进化树分析表明，TGEV 株分为 3 个聚类。TGEV TS 与美洲 Miller 聚类株密切相关，但 5'非翻译区（NTR）序列与美洲 Purdue 聚类株密切相关。在不同细胞类型中的连续传代培养表明，TGEV TS 毒力在 PK-15 细胞中传代五十次后可减弱，而猪肾细胞系 IB-RS-2（IBRS）不适合 TGEV 株 TS 的培养。
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