Gregoraszczuk E L
Department of Animal Physiology, Jagiellonian University, Kracow/Poland.
Exp Clin Endocrinol. 1990 Nov;96(2):234-7. doi: 10.1055/s-0029-1211014.
The extent of progesterone and estradiol secretion by isolated either large or small luteal cells was examined during incubation of separate cell types in medium without and with 100 ng/ml PRL. Small and large luteal cells were isolated from early corpora lutea of pig by means of Ficoll continuous gradient. The amount of progesterone released by large cells into the incubation medium was 5 fold higher than that by the small ones. In the presence of PRL large cells secreted more progesterone (1.5 fold) than the control ones. We did not observe any stimulatory effect of prolactin on progesterone secretion by small luteal cells. On the other hand, prolactin added to the medium decreased estradiol production by both cell types.
在分别培养大黄体细胞和小黄体细胞的过程中，研究了在不含和含有100 ng/ml催乳素（PRL）的培养基中，分离出的大、小黄体细胞分泌孕酮和雌二醇的程度。通过Ficoll连续梯度法从猪的早期黄体中分离出小黄体细胞和大黄体细胞。大细胞释放到培养液中的孕酮量比小细胞高5倍。在有PRL存在的情况下，大细胞分泌的孕酮比对照组多（1.5倍）。我们未观察到催乳素对小黄体细胞分泌孕酮有任何刺激作用。另一方面，向培养基中添加催乳素会降低两种细胞类型的雌二醇分泌量。
