Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
J Am Chem Soc. 2010 Nov 10;132(44):15499-501. doi: 10.1021/ja1067806.
A protease from ribosomal peptide biosynthesis macrocyclizes diverse substrates, including those resembling nonribosomal peptide and hybrid polyketide-peptide products. The proposed mechanism is analogous to thioesterase-catalyzed chemistry, but the substrates are amide bonds rather than thioesters.
核糖体肽生物合成中的一种蛋白酶使多种底物大环化,包括那些类似于非核糖体肽和杂合聚酮肽产物的底物。所提出的机制类似于硫酯酶催化的化学,但底物是酰胺键而不是硫酯。