利用海洋天然产物发现一种能催化多种底物进行肽大环化的蛋白酶。
Using marine natural products to discover a protease that catalyzes peptide macrocyclization of diverse substrates.
作者信息
Lee Jaeheon, McIntosh John, Hathaway Brian J, Schmidt Eric W
机构信息
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
出版信息
J Am Chem Soc. 2009 Feb 18;131(6):2122-4. doi: 10.1021/ja8092168.
Abstract
Small N-C terminally cyclic ribosomal peptides are common in nature, yet the mechanisms underlying their biosyntheses remain largely unknown. We recently identified candidate peptide cyclase genes in the metagenomes of marine animals. Here we report that PatG, a protease discovered in this analysis, cleaves variables, short peptides out of a precursor protein, and cyclizes these peptides in vivo and in vitro.
摘要
小的N端环状核糖体肽在自然界中很常见，但其生物合成的潜在机制在很大程度上仍不清楚。我们最近在海洋动物的宏基因组中鉴定出了候选肽环化酶基因。在此，我们报告在该分析中发现的一种蛋白酶PatG，能从一种前体蛋白中切割出可变的短肽，并在体内和体外将这些肽环化。
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