商业 PCR 主混合物被贝氏柯克斯体 DNA 污染。
Contamination of commercial PCR master mix with DNA from Coxiella burnetii.
机构信息
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, and Radboud University Nijmegen Medical Center, Weg door Jonkerbos 100, 6532 SZ Nijmegen, Netherlands.
出版信息
J Clin Microbiol. 2010 Dec;48(12):4634-5. doi: 10.1128/JCM.00464-10. Epub 2010 Oct 20.
Abstract
Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.
摘要
室内诊断实时 PCR 检测 Q 热的污染可追溯到市售的 PCR 主混合液。据证实，这种主混合液中含有贝氏柯克斯体的 DNA，可能是由于使用了牛血清白蛋白等动物来源的化合物。
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