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商业 PCR 主混合物被贝氏柯克斯体 DNA 污染。

Contamination of commercial PCR master mix with DNA from Coxiella burnetii.

机构信息

Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, and Radboud University Nijmegen Medical Center, Weg door Jonkerbos 100, 6532 SZ Nijmegen, Netherlands.

出版信息

J Clin Microbiol. 2010 Dec;48(12):4634-5. doi: 10.1128/JCM.00464-10. Epub 2010 Oct 20.

DOI:10.1128/JCM.00464-10
PMID:20962145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3008432/
Abstract

Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.

摘要

室内诊断实时 PCR 检测 Q 热的污染可追溯到市售的 PCR 主混合液。据证实，这种主混合液中含有贝氏柯克斯体的 DNA，可能是由于使用了牛血清白蛋白等动物来源的化合物。

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本文引用的文献

1
Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii DNA in serum.不同提取和实时 PCR 方法检测血清中贝氏柯克斯体 DNA 的实验室间评估。
J Clin Microbiol. 2010 Nov;48(11):3923-7. doi: 10.1128/JCM.01006-10. Epub 2010 Sep 8.
2
Prevalence of Coxiella burnetii antibodies in Danish dairy herds.丹麦奶牛场中贝氏柯克斯体抗体的流行情况。
Acta Vet Scand. 2010 Jan 21;52(1):5. doi: 10.1186/1751-0147-52-5.
3
Sustained intensive transmission of Q fever in the south of the Netherlands, 2009.2009年荷兰南部Q热的持续密集传播。
Euro Surveill. 2009 May 14;14(19):19210. doi: 10.2807/ese.14.19.19210-en.
4
Comparison of Coxiella burnetii shedding in milk of dairy bovine, caprine, and ovine herds.奶牛、山羊和绵羊群体乳汁中伯氏考克斯氏体排出情况的比较。
J Dairy Sci. 2007 Dec;90(12):5352-60. doi: 10.3168/jds.2006-815.
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Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.实时通用16S rRNA聚合酶链反应的污染和敏感性问题。
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