University of Turin, Department of Exploitation and Protection of the Agricultural and Forestry Resources (DiVaPRA), Plant Genetics and Breeding, Via Grugliasco (TO), Italy.
Genome. 2010 Oct;53(10):805-15. doi: 10.1139/g10-073.
An anther-derived doubled haploid (DH) population and an F2 mapping population were developed from an intraspecific hybrid between the eggplant breeding lines 305E40 and 67/3. The former incorporates an introgressed segment from Solanum aethiopicum Gilo Group carrying the gene Rfo-sa1, which confers resistance to Fusarium oxysporum; the latter is a selection from an intraspecific cross involving two conventional eggplant varieties and lacks Rfo-sa1. Initially, 28 AFLP primer combinations (PCs) were applied to a sample of 93 F2 individuals and 93 DH individuals, from which 170 polymorphic AFLP fragments were identified. In the DH population, the segregation of 117 of these AFLPs as well as markers closely linked to Rfo-sa1 was substantially distorted, while in the F2 population, segregation distortion was restricted to just 10 markers, and thus the latter was chosen for map development. A set of 141 F2 individuals was genotyped with 73 AFLP PCs (generating 406 informative markers), 32 SSRs, 4 tomato RFLPs, and 3 CAPS markers linked to Rfo-sa1. This resulted in the assignment of 348 markers to 12 major linkage groups. The framework map covered 718.7 cM, comprising 238 markers (212 AFLPs, 22 SSRs, 1 RFLP, and the Rfo-sa1 CAPS). Marker order and inter-marker distances in this eggplant map were largely consistent with those reported in a recently published SSR-based map. From an eggplant breeding perspective, DH populations produced by anther culture appear to be subject to massive segregation distortion and thus may not be very efficient in capturing the full range of genetic variation present in the parental lines.
一个花粉衍生的加倍单倍体（DH）群体和一个 F2 作图群体是由茄子育种系 305E40 和 67/3 之间的种内杂种开发的。前者包含来自茄属 Solanum aethiopicum Gilo Group 的一个渐渗片段，该片段携带基因 Rfo-sa1，可赋予对尖孢镰刀菌的抗性；后者是来自涉及两个常规茄子品种的种内杂交的选择，缺乏 Rfo-sa1。最初，使用 28 个 AFLP 引物组合（PC）对 93 个 F2 个体和 93 个 DH 个体的样本进行了分析，从中鉴定出 170 个多态性 AFLP 片段。在 DH 群体中，这些 AFLP 中的 117 个以及与 Rfo-sa1 紧密连锁的标记的分离明显失真，而在 F2 群体中，分离失真仅限于仅 10 个标记，因此选择后者进行图谱开发。一组 141 个 F2 个体用 73 个 AFLP PC（产生 406 个信息标记）、32 个 SSR、4 个番茄 RFLP 和 3 个与 Rfo-sa1 连锁的 CAPS 标记进行了基因型分析。这导致将 348 个标记分配到 12 个主要连锁群中。框架图谱覆盖 718.7 cM，包含 238 个标记（212 个 AFLP、22 个 SSR、1 个 RFLP 和 Rfo-sa1 CAPS）。在这个茄子图谱中，标记的顺序和标记之间的距离与最近发表的基于 SSR 的图谱中报道的基本一致。从茄子育种的角度来看，花药培养产生的 DH 群体似乎受到大规模分离失真的影响，因此可能无法非常有效地捕获亲本群体中存在的全部遗传变异。
