Dybvig K, Khaled M
Department of Comparative Medicine, University of Alabama, Birmingham 35294.
Plasmid. 1990 Sep;24(2):153-5. doi: 10.1016/0147-619x(90)90018-8.
Plasmids have rarely been detected in organisms constituting the genus Mycoplasma. Recently, the isolation of a cryptic plasmid from Mycoplasma mycoides subsp. mycoides has been described, and we report here the isolation of a second cryptic plasmid from this species. Restriction map and Southern blot analyses show that the second plasmid is distinct from the previously described plasmid, although a limited region of homology was detected. The availability of mycoplasmal cryptic plasmids may lead to the development of cloning vectors that replicate in these organisms.
